Simulation of Confocal Microscopy with Structured Illumination and Signal Evaluation

Philipp Heidingsfelder1,a, Jun Gao2 and Ing. Peter Ott1,b

1Institute for Mechatronics, Optical Engineering Lab, Heilbronn University, Max-Planck-Str. 39, D-74081 Heilbronn, Germany.

2School of Computer and Information, Hefei University of Technology Tunxi Road No.193, CN- 230009 Hefei, Anhui, China.


In this paper we present the simulation of a digital confocal microscope combined with a slit pattern illumination. First we show shortly the function and principle of confocal microscopes and subsequently our digital confocal microscope we want to realise and simulate. For the optic design we create a wave-optical model of the microscope in Matlab. The model is based on Fourier optics and allows us to optimize the illumination pattern of the confocal microscope by simulating the light intensity on the pixels of the CCD-camera. Furthermore this model permits to simulate different algorithms for the calculation of the focal-depth response and to determine the z-resolution of the microscope. The model, the theory, the results of the simulation and the comparison to the literature are presented in this document.

Keywords: Confocal microscope, Structured illumination, Digital microscope, 3D-microscopy, Pattern illumination, Fringe pattern, Focal-depth response, Waveoptical model, Fourier-optics.

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