Biomachining has been developed over several years to make use of metabolic activity of microorganisms for micro fabrication. Many researchers have reported biomachining capabilities for micromachining. In present study, the influence of different process parameters such as temperature, shaking rate, and FeSO4 concentration etc. on metal removal rate (MRR) is investigated by employing acidithiobacillus ferrooxidans bacteria as a cutting tool for biomachining of iron and optimum parameters are selected on the basis of experimental results. It is observed that process parameters can be tuned to get higher MRR. Magnetic stirring and continual culture inoculation techniques have been developed to obtain better and constant metal removal rate of biomachining respectively.