In this work, we aim to investigate if permeability of fluids will be affected by the distribution of porous hole sizes in open cell culture with constant porosity. Many tissue scaffolds are open cell culture which allows cells to attach on and grow with the constant flow of nutrients to maintain the tissue culture. The lack of nutrients flowing into a Three-Dimensional (3D) tissue culture is one of the several issues faced in maintaining cell culture. We are proposing a method to test the permeability of various open cell porous structures with constant porosity value but with varying distribution of porous hole sizes. A basic open cell structure was obtained from researched work and with the help of 3D printing, the porous structure was custom built for the intended criteria. A suitable set of experiment was chosen to conduct the permeability test which led to the design of the apparatus to fit the porous structures. The porous structures and apparatus were drawn using the Computer Aided Design (CAD) software, Solidworks. The models were then fabricated using 3D printing technology. The Stereolithography (SLA) process was selected for the production of the porous structures as it gives a good surface finishing which is critical for the test of permeability. The Fused Deposition Modelling (FDM) process was chosen for the fabrication of the apparatus as the printer has a bigger building platform to accommodate the size of the apparatus as compare to the SLA. The experimental results were then analysed and concluded to prove if the porous hole sizes distribution in similar open cell porous structures has affected permeability. The experiment results showed that the porous hole sizes distribution in various porous models with similar porosity value affected permeability. With this knowledge, we can further control the flow rate of nutrients by manipulating the pore size to help increase or decrease the growth rate of cells.