Jatropha Curcas Biodiesel is one of the many alternative fuel options that can help in reducing oil dependence. It is derived from vegetable oil or animal fats that can be an additive to or entirely replace conventional Petroleum diesel fuel. Transesterification reaction of Jatropha curcas oil with methanol to oil ratio was 20 %w/w investigated by using of sodium hydroxide catalyst to oil ratio 1 %w/w. An experiment has been carried out in the clamp on reactor - type reaction vessel with tube diameter 60 mm, and processing time variations with 5, 10 and 15 minute. After transesterification process, tri, di, mono, free and total glycerol were determined with Gas Chromatography. An Attenuated Total Reflection Graph (ATR) was used to monitor FAME and Triglycerides (TGs) formation. The tri, di and mono less than 1 %by clamp-on tubular reactor with tube diameter 60 mm in 7 minute. This process can reduce processing time by almost 80 %than that of conventional one.