Recent advances in acoustic manipulation technology has seen the emergence various approaches and device designs. One of which, the counter propagating wave approach, relies on matched transducers in reducing reflection coefficient to achieve manipulation. A new design using unmatched transducers based on counter propagating wave approach with high manipulation capability is presented. Fabrication of devices is simplified by omission of the matching layer. Manipulation is achieved by tuning in to the through thickness resonances of the opposing pair of transducers at which reflections are low enough to allow sufficiently strong controllable acoustic trapping to be generated. An application using one such device is also presented where two types of cells are patterned independently on the same dish while maintaining high viability.