The study of flapping mechanism of insect wing during flight can provide the important information for the design of the micro aerial vehicle (MAV). The larger size of the dragonfly wing which increases the stiffness significantly in a lightweight structure has been paid much attention in the past studies of the aerodynamics, deformation behaviors and vibration analysis. In most of these studies the dragonfly wings were assumed as ahomogeneous material. In fact, the dragonfly wing is a nonhomogeneous material composed of membrane, vein and pterostigma. Thus, the nanoindentational technique was used to measure the nanomechanical properties locally such as hardness and elastic modulus herein. To verify the availability of the measured properties, the vibration modal analysis was also conducted by using the precise three-dimensional numerical model established by inverse engineering. The results indicate the deformation of the dragonfly wing is dominated by the vein structure to sustain the membrane light structure and support the aerodynamic forces.